Journal: Investigative ophthalmology & visual science

Article Title: Ocular drug delivery targeting the retina and retinal pigment epithelium using polylactide nanoparticles.

doi: 10.1167/iovs.02-1068

Figure Lengend Snippet: FIGURE 9. Immunohistochemistry of the retina and the ciliary body at 6, 24, and 48 hours after injection. Infiltrating OX42- and ED1-positive cells are green. The NPs fluoresced in red. OX42- or ED1-positive cells engulfing the NPs were costained and fluoresced in yellow.

Article Snippet: Sections were then incubated in humidified atmosphere at room temperature for 1 hour with the following specific antibodies at 1:50 (in PBS, 1% skimmed milk, 0.5% Triton): monoclonal mouse anti-rat ED1 staining myeloid cells such as macrophages (Serotec, Oxford, UK); monoclonal mouse anti-rat OX42/CD11b staining microglia, dendritic cells, granulocytes, and macrophages (Serotec); polyclonal rabbit anti-bovine glial fibrillary acidic protein (GFAP) staining astrocytes and activated Müller cells (DAKO, Glostrup, Denmark); and monoclonal mouse anti-rat anti-pan cytokeratin (CytoK) staining epithelial cells.

Techniques: Immunohistochemistry, Injection

Journal: Pharmaceutical Biology

Article Title: Hepatoprotective effect of peony total glucosides and the underlying mechanisms in diabetic rats

doi: 10.1080/13880209.2017.1390589

Figure Lengend Snippet: Immunostaining of ED-1 positive cell in the liver. (A) Normal; (B) control diabetic; (C) diabetic + TGP 50 mg/kg; (D) diabetic + TGP 100 mg/kg; (E) diabetic + TGP 200 mg/kg. Original magnification 100×.

Article Snippet: Mouse anti-macrophage monoclonal (ED-1) antibody was from Abcam Biotechnology (Abcam, Cambridge, UK).

Techniques: Immunostaining

Journal: Histochemistry and Cell Biology

Article Title: Myeloperoxidase and elastase are only expressed by neutrophils in normal and in inflammed liver

doi: 10.1007/s00418-011-0787-1

Figure Lengend Snippet: Immunhistochemical detection of myeloperoxidase (MPO), neutrophil elastase (NE) and ED1 cells in rat liver 6 h after one single oral dose of CCl 4 -adminstration induced rat liver injury. MPO + cells ( a ), ED1 + cells ( b ) and NE + cells ( c ) in control rat liver. MPO + cells ( d ), ED1 + cells ( e ) and NE + cells ( f ) at 6 h after treatment. The lower panel shows MPO + cells ( g ), ED1 + cells ( h ) and NE + cells ( i ) at 24 h after treatment. Original magnification, ×100. G original magnification, ×200 173 × 116 mm (300 × 300 DPI)

Article Snippet: Mouse monoclonal antibody ED1 against rat monocyte/macrophage was purchased from Serotec (Düsseldorf, Germany).

Techniques: Control

Journal: Histochemistry and Cell Biology

Article Title: Myeloperoxidase and elastase are only expressed by neutrophils in normal and in inflammed liver

doi: 10.1007/s00418-011-0787-1

Figure Lengend Snippet: MPO + and ED1 + cells in rat liver after γ -Irradiation induced rat liver injury. ED1 + cells ( a ) and MPO + cells ( b ) in control rat liver. ED1 + cells ( c ) and MPO + cells ( d ) at 3 h after γ -Irradiation. ED1 + cells ( e ) and MPO + cells ( f ) at 24 h after γ -Irradiation. Original magnification, ×100 173 × 174 mm (300 × 300 DPI)

Article Snippet: Mouse monoclonal antibody ED1 against rat monocyte/macrophage was purchased from Serotec (Düsseldorf, Germany).

Techniques: Irradiation, Control

Journal: Histochemistry and Cell Biology

Article Title: Myeloperoxidase and elastase are only expressed by neutrophils in normal and in inflammed liver

doi: 10.1007/s00418-011-0787-1

Figure Lengend Snippet: Double staining of liver sections with monoclonal antibodies directed against MPO or NE ( red ) and monoclonal antibody against ED1 ( green ) followed by fluorescence immunodetection in sections of rat liver at different time points after CCl 4 -administration. a NE + or ED1 + cells in CCl4-induced liver injury at 0 h. b MPO + or ED1 + cells in CCl 4 -induced liver injury at 0 h. c NE + or ED1 + cells in CCl 4 -induced liver injury 24 h after administration. d MPO + or ED1 + cells in CCl 4 -induced liver injury 24 h after administration. Original magnification, ×100 173 × 130 mm (300 × 300 DPI)

Article Snippet: Mouse monoclonal antibody ED1 against rat monocyte/macrophage was purchased from Serotec (Düsseldorf, Germany).

Techniques: Double Staining, Bioprocessing, Fluorescence, Immunodetection

Journal: Histochemistry and Cell Biology

Article Title: Myeloperoxidase and elastase are only expressed by neutrophils in normal and in inflammed liver

doi: 10.1007/s00418-011-0787-1

Figure Lengend Snippet: Immunofluorescence staining of liver sections after γ -Irradiation at different time points with monoclonal antibodies directed against MPO ( red ) and monoclonal antibody against ED1 ( green ). MPO + cells ( a ) or ED1 + cells ( b ) in control liver. MPO + cells ( c ) and ED1 + cells ( d ) at 3 h after γ -Irradiation. MPO + cells ( e ) and ED1 + cells ( f ) at 6 h after γ -Irradiation. Original magnification, ×100 15 × 16 mm (600 × 600 DPI)

Article Snippet: Mouse monoclonal antibody ED1 against rat monocyte/macrophage was purchased from Serotec (Düsseldorf, Germany).

Techniques: Immunofluorescence, Staining, Irradiation, Bioprocessing, Control

Journal: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association

Article Title: Macrophages contribute to the initiation of ischaemic acute renal failure in rats.

doi: 10.1093/ndt/gfk047

Figure Lengend Snippet: Fig. 1. Effect of LC on percentage peripheral blood monocytes and liver macrophages. Rats were injected with either LV or LC via tail vein. At 24 h later, blood samples and liver tissue were obtained to determine blood monocyte–macrophage depletion. (A) Percentage blood monocytes, (B) LV and (C) LC, liver macrophages (ED-1 staining, 100). (D) Number of ED-1-positive cells per HPF (200) in liver. *P<0.05 compared with LV, n ¼ 5 animals per group.

Article Snippet: Immunohistochemical detection of monocytes–macrophages was performed on paraformaldehyde (4%)-fixed and paraffinembedded kidney and liver tissue sections using the mouse monoclonal antibody against ectodysplasin-1 (ED-1) (Serotec, UK).

Techniques: Injection, Staining

Journal: Journal of dairy science

Article Title: M1 polarization of hepatic macrophages in cows with subclinical ketosis is an important cause of liver injury.

doi: 10.3168/jds.2024-25500

Figure Lengend Snippet: Figure 3. Hepatic macrophage counts and associated genetic changes in healthy cows and dairy cows with SCK. (A) Hepatic protein levels of Cluster of Differentiation 68 (CD68) in healthy (n = 6) and SCK (n = 6) cows. (B) Quantification of hepatic CD68 protein levels. (C) Relative hepatic mRNA levels of CD68. (D) Relative mRNA expression levels of (i) C-type lectin domain family 4 member F (CLEC4F) and (ii) small inducible cytokine subfamily B (Cys-X-Cys), member 10 (CXCL10) in the liver of healthy (n = 6) and SCK (n = 6) cows. (E) Levels of (i) CXCL1 and (ii) CCL2 in the liver of healthy (n = 6) and SCK (n = 6) cows. (F) Representative images (scale bar = 50 μm) of immunofluorescence for CD68 (pink) and DAPI (sky blue) in bovine liver. (G) Quantification of MFI of CD68 in the liver of healthy (n = 6) and SCK (n = 6) cows. Data are presented as mean ± SEM. Statistical differences were assessed by t-test.

Article Snippet: The primary antibodies included: rabbit anti-NFκB polyclonal antibody (1:100; bs-0465R, Bioss), rabbit anti- CD68 polyclonal antibody (1:100; bs-1432R, Bioss), mouse anti- CD68 monoclonal antibody (1:100; NB600–985, NOVUS), rabbit anti-CD86 polyclonal antibody (1:100; bs-1035R, Bioss), rabbit anti-CD206 Recombinant antibody (1:100; 81525–1-RR, Proteintech).

Techniques: Expressing, Immunofluorescence

Journal: Journal of dairy science

Article Title: M1 polarization of hepatic macrophages in cows with subclinical ketosis is an important cause of liver injury.

doi: 10.3168/jds.2024-25500

Figure Lengend Snippet: Figure 4. Hepatic macrophages in SCK cows are predominantly M1 polarized. (A) Representative Western blots of inducible nitric oxide sythase (iNOS), CD86, IL-1β and β-actin in the liver of healthy (n = 6) and SCK (n = 6) cows. (B) Quantification of protein levels of (i) iNOS, (ii) CD86 and (iii) IL-1β. (C) Relative mRNA expression levels of iNOS, CD86, IL-1β, IL-6, IL-12b and chemokine (C-C motif) ligand 3 (CCL3) in the liver of healthy (n = 6) and SCK (n = 6) cows. (D) Levels of IL-1β in the liver of healthy (n = 6) and SCK (n = 6) cows. (E) Representative images (scale bar = 20 μm) of immunofluorescence for CD68 (green), CD86 (red) and DAPI (blue) in bovine liver. (F) Quantification of MFI of CD86 in the liver of healthy (n = 6) and SCK (n = 6) cows. (G) Quantification of the number of CD68+/CD86+ positive cells in the liver of healthy (n = 6) and SCK (n = 6) cows. (H) Representative images (scale bar = 50 μm) of immunofluorescence for IL-1β (yellow), iNOS (red) and DAPI (blue) in bovine liver. (I) Quantification of MFI of (i) IL-1β and (ii) iNOS in the liver of healthy (n = 6) and SCK (n = 6) cows. Data are presented as mean ± SEM. Statistical differences were assessed by t-test.

Article Snippet: The primary antibodies included: rabbit anti-NFκB polyclonal antibody (1:100; bs-0465R, Bioss), rabbit anti- CD68 polyclonal antibody (1:100; bs-1432R, Bioss), mouse anti- CD68 monoclonal antibody (1:100; NB600–985, NOVUS), rabbit anti-CD86 polyclonal antibody (1:100; bs-1035R, Bioss), rabbit anti-CD206 Recombinant antibody (1:100; 81525–1-RR, Proteintech).

Techniques: Western Blot, Expressing, Immunofluorescence

Journal: Journal of dairy science

Article Title: M1 polarization of hepatic macrophages in cows with subclinical ketosis is an important cause of liver injury.

doi: 10.3168/jds.2024-25500

Figure Lengend Snippet: Figure 5. Hepatic macrophage M2 polarization decreased in SCK cows. (A) Representative Western blots of CD206, IL-10 and β-actin in the liver of healthy (n = 6) and SCK (n = 6) cows. (B) Quantification of hepatic (i) CD206 and (ii) IL-10 protein levels. (C) Relative hepatic mRNA levels of CD206, IL-10 and arginase 1 (Arg1). (D) Levels of IL-10 in the liver of healthy (n = 6) and SCK (n = 6) cows. (E) Representative images (scale bar = 20 μm) of immunofluorescence for CD68 (green), CD206 (pink) and DAPI (blue) in bovine liver. (F) Quantification of MFI of CD206 in the liver of healthy (n = 6) and SCK (n = 6) cows. (G) Quantification of the number of CD68+/ CD206+ positive cells in the liver of healthy (n = 6) and SCK (n = 6) cows. Data are presented as mean ± SEM. Statistical differences were assessed by t-test.

Article Snippet: The primary antibodies included: rabbit anti-NFκB polyclonal antibody (1:100; bs-0465R, Bioss), rabbit anti- CD68 polyclonal antibody (1:100; bs-1432R, Bioss), mouse anti- CD68 monoclonal antibody (1:100; NB600–985, NOVUS), rabbit anti-CD86 polyclonal antibody (1:100; bs-1035R, Bioss), rabbit anti-CD206 Recombinant antibody (1:100; 81525–1-RR, Proteintech).

Techniques: Western Blot, Immunofluorescence